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KMID : 0620920000320010042
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Experimental & Molecular Medicine
2000 Volume.32 No. 1 p.42 ~ p.46
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Regulation of bcl-2 family in hydrogen peroxide-induced apoptosis in human leukemia HL-60 cells
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Jung Hwa Lee/Jung Eun Lee
Jeong Won Sohn/Jung Hwa Lee/Kwang Chul Lee/Chang Sung Son/Young Chang Tockgo
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Abstract
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Numerous types of cells have been shown to undergo apoptosis when exposed to oxidant agent such as hydrogen peroxide. In order to understand the functional relationship between the anti- and pro-apoptotic regulatory proteins in the cells under oxidant stress, we have studied the level of expression of apoptosis regulatory proteins, bcl-2 and bax, in human leukemia HL-60 cells. The exposure of HL-60 cells to different concentrations of H2O2 for 6 h resulted in a typical apoptosis of the cells as characterized by flow cytometry, cell cycle analysis, and DNA fragmantation. There was a block in G1 to S transition and apoptotic cells were mainly derived from S and G2 cells. Kinetic study demonstrated that the levels of both bcl-2-mRNA and -protein expression were decreased with the progression of cellular apoptosis whereas the level of bax-mRNA was unchanged but the expressed bax-protein was not detectable. Cycloheximide, a nonspecific translation inhibitor, did not prevent the hydrogen peroxide-mediated apoptosis in HL-60 cells. These results suggest that the regulation of bcl-2, but not of bax are important factor in the oxidative stress-induced apoptosis in HL-60 cells.
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KEYWORD
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hydrogen peroxide, apoptosis, HL-60 cell, Bcl-2, cell cycle,
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